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. 2017 Jun 22;7:4055. doi: 10.1038/s41598-017-04419-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Range of GCN values across all the experimental conditions and technical replicates. Boxplot (min. to max. and median values) of the range of GCN values for all 97 amplicons included in the library reparation of the AmpliSeq customized panel for all the experimental conditions. (a) Represents the data from library preparation replicate #1, (b) or #2, (c) or #3. A two-tailed, Wilcoxon matched-pairs signed rank test was used to assess the statistical significance of the difference between different experimental conditions. The range of normal gene dosage is 1 ± 0.25. *Indicates p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001. FFPE: formalin-fixed and paraffin embedded tissue, WGA: Whole genome amplification, GCN: Gene copy number.