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. 2017 Jun 22;7:4055. doi: 10.1038/s41598-017-04419-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Biased variants analyzed by nucleotide base substitution, variant type, and the used WGA kit. Histograms of number of variants biased in (a) all the altered variants, (b) germline plus somatic variants or (c) on false-positive variants created during WGA. The data includes the three replicates of lung cancer #1, as well as the lung cancer #2 and esophageal cancer #1 sets. The statistical significance was calculated as described in methods. *Indicates p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001. FFPE: formalin-fixed and paraffin embedded tissue, WGA: Whole genome amplification.