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. 2017 May 15;10(4):896–909. doi: 10.1111/1751-7915.12728

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© 2017 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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The chemical inactivation of LdtR modifies the expression profile of several functional categories in L. crescens. The expression levels of a subset of genes identified in (A) RNA‐seq experiments, or (B) RegPredict, were assessed to monitor the transcriptional activity of LdtR upon inactivation by benzbromarone (blue boxes) or phloretin (orange boxes). Grey boxes represent control conditions. The horizontal line in each box plot represents the mean value. The relative expression values of each gene were obtained by normalizing their levels against the expression levels of 16S rRNA of L. crescens. The changes in expression for every gene due to the addition of benzbromarone or phloretin displayed statistical significance (P < 0.05) compared to control conditions according to ANOVA. The experiments were performed in quadruplicates.