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. 2017 Jun 15;10(4):958–968. doi: 10.1111/1751-7915.12736

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© 2017 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Relative expression of the genes of the sorbicillinoid biosynthesis gene cluster.

A. Schematic representation of the gene cluster: Pc21 g05050 (sorR1; transcriptional factor), Pc21 g05060 (sorC; monooxygenase), Pc21 g05070 (sorB; non‐reduced polyketide synthase), Pc21 g05080 (sorA; highly reduced polyketide synthase), Pc21 g05090 (sorR2; transcriptional factor), Pc21 g05100 (sorT; MFS transporter) and Pc21 g05110 (sorD; oxidase).

B. Quantitative real‐time PCR analysis in sorbicillinoid gene cluster expression in strains with individual deleted sorA, sorB, sorC, sorD and sorT.

C. sorR1 and sorR2 genes.

D. qPCR analysis in strains and overexpressed sorR1 and sorR2. Samples were taken at day 1 (grey bars), 3 (white bars) and 5 (black bars). Data shown as fold change relative to P. chrysogenum DS68530Res13 strain.