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. 2017 Apr 18;10(4):873–885. doi: 10.1111/1751-7915.12716

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© 2017 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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(A) UPLC analysis of Streptomyces sp. NTK937 wild‐type (WT) and mutant strains ΔcbxA, ΔentC and ΔcbxA/ΔentC. (B) UPLC analysis mutant strain ΔcbxR. (C) UPLC analysis of mutant strains carrying individual inactivation of each structural gene of the caboxamycin biosynthesis cluster. Extracts were obtained from cultures grown in R5A medium for 7 days. UPLC chromatograms were analysed at 330 nm. Labelled peaks correspond: caboxamycin A (1), O‐methylcaboxamycin (2), 3′‐hydroxycaboxamycin (3).