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. 2016 Nov 14;10(4):761–772. doi: 10.1111/1751-7915.12435

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© 2016 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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FISH‐CLSM of biofilm cryosections. The water‐biofilm interface is oriented to the lower left. In all images, anammox bacteria (Amx820) are in red and nonspecific bacteria (EUB‐mix) are in white‐grey.

A. In green: AOB within the Nitrosomonas europaea/eutropha cluster (Nse1472).

B. In green: AOB within Nitrosospira (Nsv443).

C. In green: NOB within Nitrospira (Ntspa662).

D. In green: Bacteria within Chloroflexi (CFX123 + GNSB941).

E. In green: Ca. Brocadia fulgida (Bfu613). Scale bar: 25 μm.