Figure 2.
Hsp90 inhibitor (17-AAG) dose response in mouse brain. Mice were injected i.c.v. with a dose response of 17-AAG or an equalized vehicle and allowed to recover for 24 h. The mice were sacrificed, and the PAG dissected, removed, and analyzed by Western blotting. Representative blots of Hsp70 and GAPDH are shown with treatment and molecular weight markers indicated. The Hsp70 signal is normalized to GAPDH and further to vehicle control. GAPDH was not altered by 17-AAG treatment. The data were reported as the mean ± S.D. Linear regression was performed on the data points, and the A50 (0.084 nmol) was calculated as described under “Experimental procedures.” The reported sample size is for experiments performed on individual mice (biological replicates) over two technical replicates on different days with different mouse cohorts by the same experimenter. Based on this experiment, 0.5 nmol of 17-AAG was chosen for further experiments.