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. 2017 May 3;292(25):10429–10443. doi: 10.1074/jbc.M117.774950

Figure 2.

Figure 2.

RNA helicase activity of DDX43 protein. A, a representative image of helicase reactions performed by incubating 0.5 nm 5′-tailed 13-bp duplex RNA substrate with increasing protein concentration (0–3 μm) at 37 °C for 15 min. NE, no enzyme. B, a representative image of helicase reactions performed by incubating 0.5 nm 5′-tailed 13-bp duplex RNA substrate and 150 nm DDX43 protein with increasing time (0–30 min) at 37 °C. C and D, representative images of helicase reactions performed by incubating 0.5 nm 3′-tailed 13-bp duplex RNA substrate (C) and 13-bp blunt end duplex RNA substrate (D) with increasing protein concentration (0–3 μm) at 37 °C for 15 min. E, quantitative analyses of RNA unwinding of DDX43 in panels A, C, and D. Data represent the mean of at least three independent experiments with S.D. indicated by error bars. F, a representative image of helicase reactions performed by incubating a 0.5 nm 5′-tailed 16-bp duplex RNA substrate with increasing protein concentrations (0–3 μm) at 37 °C for 15 min. RNA is in black.