Figure 4.

Ppp1r3b-deficient livers undergo rapid switching from glycolysis to gluconeogenesis. A, ad libitum chow-fed liver lysates were used for measurement of transcript levels of glycolytic and gluconeogenic genes (Gck, Gpi1, Pfk1, Aldob, Tpi1, Gapdh, Pgk1, Pgam1, Eno1, Pklr, Pepck, and G6pc (n = 6–9/genotype). B, 8-h fasted liver lysates were used for measurement of transcript levels of the above glycolytic and gluconeogenic genes (n = 5/genotype). The results were replicated in at least two independent experiments. The data are expressed as the means ± S.E. Significance was determined in all panels by unpaired Student's t test. *, p < 0.05; **, p < 0.005; ***, p < 0.0001. n.s, not significant.