Figure 2.

Cellular protein C processing is abrogated by incubation of cells with α1-PDX. The left panel represents the production levels of V5-tagged α1-PDX in COS-1 cells. In the right panel, full-length protein C (mProtein C) (tagged with hexa-His at the C terminus) was co-expressed in COS-1 cells with either empty vector or that encoding human furin, and the cells were then incubated for 16 h with media containing α1-PDX, RVKR (50 μm), or D6R (20 μm). The media were then analyzed for protein C by Western blotting using anti-His tag antibody. Quantifications were done using image analysis and are representative of two independent experiments.