Figure 5.

Hepatic expression of the target genes for miR-194 and miR-192 in Hnf4a^ΔH mice. A, real-time PCR from total liver RNA of Hnf4a^ΔH and Hnf4a^f/f mice. Known (A) and new (B) mRNA targets for miR-194 and known and new targets (C) for miR-192. Error bars represent S.D. Data are mean ± S.D. of five independent mice. *, p < 0.05. D, Western blot of CLN4B and ALCAM protein from livers of Hnf4a^ΔH and Hnf4a^f/f mice (n = 3 for each genotype). CLN4B and ALCAM expression normalized by total protein in Hnf4a^ΔH was presented as relative to Hnf4a^f/f mice. CBB, Coomassie Brilliant Blue.