Figure 3.

The N and C termini of TDP-43 are required for promotion of tau exon 10 inclusion. A, schematic diagram of TDP-43 truncations used. B, the truncation mutants of TDP-43 tagged with HA were expressed in HeLa cells for 48 h and immunostained with monoclonal anti-HA followed by FITC-conjugated goat anti-mouse IgG (green). Hoechst stain (blue) was used to stain nuclei. C, percentage of cells with TDP-43 aggregates in TDP-43-transfected cells. Deletion of the N or C terminus of TDP-43 was found to promote its cytoplasmic aggregation. D, deletion mutants of TDP-43 were co-transfected with pCI/SI9-LI10 into HEK-293FT cells. The splicing products of tau exon 10 were determined. Deletion of either the N or C terminus of TDP-43 was found to abolish its function in promoting tau exon 10 inclusion. The data are presented as mean ± S.D. (error bars) (n = 3). *, p < 0.05; ***, p < 0.001; #, p < 0.05; ###, p < 0.001; &, p < 0.05; &&&, p < 0.001; @@@, p < 0.001; *, versus control; #, versus TDP-43^FL; &, versus TDP-43^1–383; @, versus TDP-43^100–414. Scale bar, 50 μm. Con, control; NES, nuclear export signal; GRD, glycine-rich domain.