Figure 4.

ALS/FTLD-U mutations of TDP-43 enhance its activity in promotion of tau exon 10 inclusion. A and B, different mutants of pCI/TDP-43·HA were co-transfected with pCI/SI9-LI10 into HEK-293FT for 48 h. The protein levels of TDP-43 and GAPDH were determined by Western blotting (A) and the splicing products of tau exon 10 were detected by RT-PCR (B). ALS/FTLD-U-related TDP-43 mutations were found to enhance tau exon 10 inclusion more effectively. C, ALS/FTLD-U mutants of TDP-43 tagged with HA were overexpressed in HeLa cells for 48 h and immunostained with monoclonal anti-HA followed by TRITC-conjugated goat anti-mouse IgG (red). Hoechst stain (blue) was used to stain nuclei. No cytoplasmic aggregation was found in cells in which ALS/FTLD-U mutations of TDP-43 were overexpressed. The data are represented as mean ± S.D. (error bars). **, p < 0.01 versus control; ##, p < 0.01 versus TDP-43. Scale bar, 50 μm. Con, control.