Table 3.

ITC binding data for BT3311 and BT3313

Protein	Ligand	Ka	ΔH	N
		m−1	kcal
BT3311	1,6-β-Glucan (fit as 1 mm)	6.5 × 104 ± 0.2	−19 × 104 ± 0	0.8 ± 0.02
BT3311	1,6-β-Glucoheptaose	2.1 × 104 ± 0.1	−14 × 104 ± 0	1.2 ± 0.02
BT3311	1,6-β-Glucohexaose	1.6 × 104 ± 0.1	−14 × 104 ± 1	1.0 ± 0.04
BT3311	1,6-β-Glucopentaose	8.3 × 103 ± 0.3	−16 × 104 ± 3	1.1 ± 0.1
BT3311	1,6-β-Glucotetraose	Lowa	Low	Low
BT3311	1,6-β-Glucobiose	NBb	NB	NB
BT3313	1,6-β-Glucan (fit as 1 mm)	5.3 × 105 ± 0.1	−36 × 104 ± 1	1.02 ± 0.04
BT3313	1,6-β-Glucoheptaose	1.5 × 104 ± 0.2	−24 × 104 ± 3	1.23 ± 0.1
BT3313	1,6-β-Glucohexaose	8.0 × 103 ± 0.7	−15 × 104 ± 2	1.38 ± 0.2
BT3313	1,6-β-Glucopentaose	3.6 × 103 ± 0.2	−15 × 104 ± 3	1.28 ± 0.2
BT3313	1,6-β-Glucotetraose	Low	Low	Low
BT3313	1,6-β-Glucobiose	NB	NB	NB

^a Low, binding too low to quantify.

^b NB, no binding was evident.