Table 4.

Kinetic parameters of BT3314 and BACOVA_00946

Enzyme	Substrate	Km	kcat	kcat/Km
		μm	min−1	μm−1 min−1
BT3314	pNP-β-d-Glca	2403 ± 379	40.82 ± 2.9	0.02
BT3314	1,3-β-Glucobiose	NDb	ND	0.18 ± 0.01
BT3314	1,4-β-Glucobiose	ND	ND	0.05 ± 0.01
BT3314	1,6-β-Glucobiose	ND	ND	5.6 ± 0.2
BT3314	1,6-β-Glucotriose	ND	ND	6.7 ± 0.6
BACOVA_00946	pNP-β-d-Glc	1052 ± 87	195.8 ± 5.5	0.19
BACOVA_00946	1,3-β-Glucobiose	ND	ND	3.73 ± 0.1
BACOVA_00946	1,4-β-Glucobiose	ND	ND	0.65 ± 0.02
BACOVA_00946	1,6-β-Glucobiose	ND	ND	38.8 ± 0.8

^a pNP-β-d-Glc, 4-nitrophenyl β-d-glucopyranoside.

^b ND, not determined.