Figure 2.

Anti-TREM2 antibody, but not Aβ or anti-TYROBP antibody (Ab), induces dose-dependent increase in the TREM2-TYROBP interaction in HEK293 cells. HEK293 cells were transfected with internal control reporter pRL-TK, TREM2-CLuc-TYROBP negative control, or our TREM2-CLuc-IRES-TYROBP-NLuc plasmid prior to stimulation with ligand. Time-course data (A, C, E, and G) were assessed for luminescence over time by AUC analysis (B, D, F, and H) in RLU for n = 3 for each group. Error bars represent S.D. ααα denotes p < 0.001 versus all other groups. * denotes p < 0.05 versus TREM2/PBS as determined by one-way ANOVA and Tukey's post-test.