Figure 4.

Anti-TREM2 antibody stimulation enhances phagocytic capability of BV-2 microglial cell line. Cells were gated based on unstained cells (A), anti-TREM2 antibody-treated cells (B), or S. aureus-treated cells (C). Cells that were treated with anti-TREM2 antibody (Ab) and S. aureus particles are shown in D. The phagocytic cell population (percentage is indicated above plot) was further separated into phagocytic Lo versus Hi populations (E–G) (52). In H, relative distributions of the phagocytic cell populations into low versus high indices are represented by FlowJo software and graphical analysis. Significant differences in low or high populations were determined by one-way ANOVA followed by Tukey's post-test. Error bars represent S.D. #, ##, and ### denote p < 0.05, 0.01, and 0.001, respectively, versus those groups indicated.