The T66M mutation in TREM2 elicits enhanced TREM2 coupling to TYROBP in HEK293 cells. HEK293 cells were transfected with TREM2-CLuc-IRES-TYROBP-NLuc plasmid prior to stimulation with ligand. Time-course data (A, C, and E) were assessed for AUC (B, D, and F) in RLU for n = 3 for each group. Significant differences were determined by one-way ANOVA followed by Tukey's post-test. Error bars represent S.D. † and ††† denote p < 0.05 and 0.001, respectively, versus unstimulated WT TREM2-transfected cells. ** and *** denote p < 0.01 and 0.001, respectively. Ab, antibody.