Figure 7.

Reduced cell surface expression of TREM2 T66M mutant. A and B, flow cytometry analysis of TREM2 signal (x axis) in untransfected (blue) and transfected cells (red) with TREM2 WT, R47H, S116C, or T66M after gating with forward and side scatter profiles for live cells (not shown) (A). The numbers in A show the percent fraction of TREM2⁺ cells in transfected cells, and B shows profiles of TREM2⁺ cells after gating in A. C and D, the same experiment was performed in permeabilized cells for untransfected (blue) and transfected cells (red). The numbers in C show the percent fraction of TREM2⁺ cells in transfected cells, and D shows profiles of TREM2⁺ cells after gating in C. Average fluorescence intensity was measured for TREM2⁺ cells for each group in unpermeabilized (E) and permeabilized conditions (F). Significant differences were determined by one-way ANOVA followed by Bonferroni post-test. Error bars represent S.D. * and ** denote p < 0.05 and 0.01 versus TREM2 WT group. ††† denotes p < 0.001 versus untransfected cells (E and F).