Figure 5.

Sialic acid transferase, essential for viral infection, is dependent on the sialic acid presentation pattern of each cell line. A, lectin staining of HAP-1, HeLa, and RD cells. HAP-1 cells displayed strong 2,6-linked sialic acid presentation as shown by staining with S. nigra lectin, whereas HeLa cells exhibited 2,3-linked sialic acid as shown by straining with MAL-I. RD cells showed strong presentation of both sialic acids. B and C, each cell line was transfected with the indicated gene-targeting sgRNA and infected by EV-D68. Surviving cells are shown stained.