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. 2017 Apr 28;292(25):10709–10722. doi: 10.1074/jbc.M116.770826

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© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 5. — AcP and CobB regulate acetylation of EcLeuRS. A, AcP but not YfiQ acetylates EcLeuRS in vitro. B, CobB removal of the acetyl moiety of EcLeuRS^Ac. C, CobB deacetylation of EcLeuRS-K619^Ac and EcLeuRS-K809^Ac. In the presence of NAM or the absence of NAD⁺, CobB is inactivated. D, incubation with CobB recovers the aminoacylation activity of EcLeuRS-K619^Ac (left panel) and EcLeuRS-K809^Ac (right panel). All experiments were conducted at least twice. When quantifying of the relative amount of AcK signal/His signal, the sample without NAD⁺ and NAM was defined as 100%.