Figure 7.

Lys residues acetylated in EcArgRS. A, crystal structure of PhArgRS complexed with PhtRNA^Arg and ANP (PDB code 2ZUE). B, schematic diagram of EcArgRS. RF, Rossmann fold. C, aminoacylation assay screening of potentially crucial Lys residues. Left panel, mutation of Lys¹²⁶ and Lys⁴⁰⁸ damages the enzymatic activities of EcArgRS. Right panel, mutation of other Lys residues has a slight negative effect on the activities EcArgRS. The results are the averages and standard deviations from three independent experiments.