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AcP and CobB regulate acetylation of EcArgRS. A, AcP acetylates EcArgRS in vitro. B, CobB removes the acetyl moiety of EcArgRSAc. C, CobB deacetylates EcArgRS-K126Ac and other acetylated variants. CobB deacetylation activity is lost in the presence of NAM or the absence of NAD+. All experiments were performed at least twice. When quantifying of the relative amount of AcK signal/His signal, the group without NAD+ and NAM was defined as 100%.
