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Kinetic parameters of EcLeuRS and derived site-specific acetylated variants for EctRNALeu in aminoacylation
All parameters are average values from three independent determinations with standard deviations. nm, nonmeasurable (too low to be measured).
| Enzymes | Km | kcat | kcat/Km | Relative catalytic efficiency |
|---|---|---|---|---|
| μm | s−1 | s−1 μm−1 | % | |
| EcLeuRS-WT | 1.2 ± 0.3 | 5.7 ± 0.3 | 4.8 | 100 |
| EcLeuRS-K619Ac | nm | nm | ||
| EcLeuRS-K624Ac | 1.3 ± 0.2 | 2.8 ± 0.3 | 2.2 | 46 |
| EcLeuRS-K809Ac | 6.7 ± 0.7 | 5.0 ± 0.4 | 0.7 | 15 |
