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Kinetic parameters of EcArgRS and derived site-specific acetylated variants for EctRNAArg in aminoacylation
All parameters are average values from three independent determinations with standard deviations. nm, nonmeasurable (too low to be measured).
| Enzymes | Km | kcat | kcat/Km | Relative catalytic efficiency |
|---|---|---|---|---|
| μm | s−1 | s−1 μm−1 | % | |
| EcArgRS-WT | 2.7 ± 0.1 | 28.4 ± 3.4 | 10.5 | 100 |
| EcArgRS-K126Ac | nm | nm | ||
| EcArgRS-K408Ac | 9.0 ± 0.6 | 6.9 ± 0.5 | 0.8 | 8 |
