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Kd values between tRNAArg and EcArgRSs determined by fluorescence quenching
All parameters are average values from three independent determinations with standard deviations.
| Enzymes | Kd | Relative value |
|---|---|---|
| μm | -fold | |
| EcArgRS-WT | 0.24 ± 0.03 | 1.0 |
| EcArgRS-K126Ac | 0.26 ± 0.02 | 1.1 |
| EcArgRS-K408Ac | 0.40 ± 0.03 | 1.7 |
