Table 3.
Oligonucleotides used in this study
| Primer name | Primer sequence |
|---|---|
| R20291_alr 138s (gBlock) | TTCCCCTCTAGAAAAAAGCTTATAATTATCCTTATATGGCCATGGTGTGCGCCCAGATAGGGTGTTAAGTCAAGTAGTTTAAGGTACTACTCTGTAAGATAACACAGAAAACAGCCAACCTAACCGAAAAGCGAAAGCTGATACGGGAACAGAGCACGGTTGGAAAGCGATGAGTTACCTAAAGACAATCGGGTACGACTGAGTCGCAATGTTAATCAGATATAAGGTATAAGTTGTGTTTACTGAACGCAAGTTTCTAATTTCGATTCCATATCGATAGAGGAAAGTGTCTGAAACCTCTAGTACAAAGAAAGGTAAGTTAGCACCATGGACTTATCTGTTATCACCACATTTGTACAATCTG |
| 5′ alr | CATGCAAAAAATAACAGTG |
| 3′ alr | TTATTTTAGCAAATAACTGTTT |
| 5′ pJS116_XbaI_P1-prom | TACGAATTCGAGCTCGGTACCCGGGGATCCTCTAGACCAGTTGTAGATTCAGAGAATA |
| 3′ alr_P1-Prom | TGCCCATGTAGGCACTGTTATTTTTTGCATCACACCTCCTACTTCAGTTT |
| 5′ P1-Prom_alr | TACAATGAATAAACTGAAGTAGGAGGTGTGATGCAAAAAATAACAGTGC |
| 3′ pJS116_XhoI_alr | CCAGTGCCAAGCTTGCATGTCTGCAGGCCTCGAGTTATTTTAGCAAATAACTGTTTATTT |
| 5′ pET_alr | GTTTAACTTTAAGAAGGAGATATACATATGCAAAAAATAACAGTGCCTACATG |
| 3′ pET_alr | ATCTCAGTGGTGGTGGTGGTGGTGCTCGAGTTTTAGCAAATAACTGTTTATTTGTAC |
| 5′ alr L39A | AAGATTTGTGGAGTAATAGCAGCTGATGCATATGG |
| 3′ alr L39A | GTCCATATGCATCAGCTGCTATTACTCCACAAAT |