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. 2017 Mar 19;63(3):279–287. doi: 10.1262/jrd.2016-160

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©2017 Society for Reproduction and Development

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)

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Fig. 4. — Different methylation levels of the spermatozoa revealed by COBRA. The percentages indicated below the gels are the methylation levels measured using a human microarray. COBRA results reflected that CpG2–5 showed different methylation levels among samples analyzed by human microarray. H1–H4, JA, JB, JC: spermatozoa; ED: epididymis; TS: testis; UC: un-cut by restriction enzyme; M: 100-bp ladder DNA size marker. White arrows indicate hypomethylation, whereas black arrows indicate hypermethylation.