Table 4. Methylation levels (%) of CpG1 and the CpG1 area in samples analyzed using different methods.
Sample | H1 | H2 | H3 | H4 | H5 | JA | JB | JC | TS |
CpG-Array | 31.3 | 33.4 | 53.1 | 59.6 | - | 86.1 | 78.5 | 26.6 | - |
CpG-BiSeq | 52.4 | 25.0 | 38.5 | 46.2 | 68.4 | 84.6 | 91.7 | 15.4 | 50.0 |
Area-BiSeq | 77.9 | 71.8 | 79.0 | 87.0 | 86.8 | 90.6 | 92.1 | 72.7 | 62.0 |
CpG-Array: The methylation level of the CpG1 analyzed using human microarray. CpG-BiSeq: The methylation level of the CpG1 analyzed using bisulfate sequencing. Area-BiSeq: The average methylation level of all 22 CpG sites including CpG1 in the area (446 bp) analyzed using bisulfate sequencing.