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. 2017 Jun 11;174(14):2287–2301. doi: 10.1111/bph.13824

Figure 5.

Figure 5

CM223‐mediated modulation of oncogenic signalling pathways and proteins of the apoptotic process in U87 and NHA. (A) Western blot analysis for p‐STAT3, total STAT3, p‐AKT, total Akt, p‐ERK and total ERK on whole cell extracts from U87MG cells, cultured for 24 h in the presence of the indicated concentrations (0–20 μM) of CM223; β‐actin was used as control of protein loading. Panel shows a representative Western blot of 3 different experiments performed with similar results. (B) Western blot analysis for PARP, caspase‐3, cleaved caspase‐3, caspase‐9, cleaved caspase‐9, full length and cleaved caspase‐7, cleaved caspase‐8, Bcl‐XL, Bcl‐2 and Bim, on whole cell extracts from cell line U87MG cultured for 24 h in the presence of the indicated concentrations (0–20 μM) of CM223. β‐actin and α‐tubulin were used as control of protein loading. Panel shows a representative Western blot of three different experiments performed with similar results. (C) Western blot analysis for caspase‐3, cleaved caspase‐3, Bcl‐XL and Bim on whole cell extracts from cell line NHA cultured for 24 h in the presence of the indicated concentrations (0–20 μM) of CM223; α‐tubulin was used as control of protein loading. Panel shows a representative Western blot of three different experiments performed with similar results.