Figure 1.

Effect of deletion of hsaD gene on M. bovis BCG. Growth rates of ΔhsaD and wildtype M. bovis BCG in MB7H9 (A) on minimal medium containing 0.05% (v·v⁻¹) tyloxapol, 0.05% (v·v⁻¹) ethanol with no additional carbon source (B) or supplemented with, 100 μg·mL⁻¹ cholesterol (C) or 100 μg·mL⁻¹ glycerol (D). The symbols show the average values of three independent experiments, and the symbols encompass the range of values obtained. The intracellular survival of ΔhsaD M. bovis BCG relative to the wild‐type in RAW264.7 cells (E) was determined as described in the Methods section. The results in (E) are the results of three independent experiments in which the mean values are plotted in a staggered plot. All symbols represent the same sampling time points (days 0, 1, 2, 3 and 4) but are shown as a cluster for clarity.