Table 2.

Effects of (A) fragment 2 and its derivatives and (B) fragment 6 and a derivative on HsaD enzymic activity and growth of mycobacteria on cholesterol as carbon source

ID	Compound	Structure	IC50 (mM)	MIC (μg·mL−1) growth on cholesterol
				M. bovis BCG	M. tuberculosis
2	3,5,‐dichloro‐benzenesulfonamide	(8).	0.41 ± 0.02	<12.5	25
8	Benzenesulfonamide	(9).	NI	NI	NI
24	3,5‐dichlorosulfanilamide	(10).	0.16 ± 0.01	25	200
27	3,5‐dichloro‐4‐hydroxybenzenesulphonic acid	(11).	0.27 ± 0.01	>200	>200
6	ethyl 3,5‐dichloro‐4‐hydroxybenzoate	(12).	0.52 ± 0.02	<12.5	<12.5
32	3,5‐dichloro‐4‐hydroxybenzoic acid	(13).	0.54 ± 0.01	<12.5	200

NI indicates no inhibition observed at 5 mM for inhibition of enzyme activity and no inhibition of growth at 200 μg·mL⁻¹ as described in the Methods section. IC₅₀ values shown as average ± SD (n = 6). See Supporting Information Table S1 for full list.