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. 2017 Jun 10;174(14):2318–2333. doi: 10.1111/bph.13837

Figure 4.

Figure 4

Effect of the 5‐HT1B antagonist SB216641, the α1‐adrenoceptor antagonist prazosin, the Gq‐inhibitor UBO‐QIC, or the Gi/o‐inhibitor PTX on ERK1/2 phosphorylation produced by 5‐HT in CHO‐K1 cells expressing human α1A‐adrenoceptors. Cells were stimulated for 5 min in the presence of increasing concentrations of 5‐HT in the absence or presence of SB216641 (300 nM, pre‐incubated for 30 min; A), prazosin (300 nM, pre‐incubated for 30 min; B), UBO‐QIC (100 nM, pre‐incubated for 30 min; C) or PTX (1 ng.mL‐1, pre‐incubated for 12–18 h; D). Each point represents mean ± SEM of n (in brackets) independent experiments. *P < 0.05 in relation to the maximum response of the control.