Figure 8.

Effect of in vivo administration of ELAC on SVZ proliferating cells. Adult mice were injected in the right lateral ventricles with ELAC (5 μM, 2 μL; n = 6) or vehicle (n = 6) and then daily injected with the cell‐division marker BrdU (120 mg·kg⁻¹) for 3 days. (A) Upper panels show fluorescence microscopy images of brain coronal sections showing the ipsilateral and contralateral SVZs of mice treated with ELAC or vehicle; sections were processed for immunohistochemical detection of BrdU⁺ nuclei (red) and ASCL1 (green). Lower panels show fluorescence microscopy images of brain coronal sections showing the ipsilateral and contralateral SVZs of mice treated with ELAC or vehicle; sections were processed for immunohistochemical detection of BrdU⁺ nuclei (red), Sox2 (green) and GFAP (blue). (B) Quantification of BrdU⁺/ASCL1⁺ cells within the SVZ of mice that had received i.c.v. injections of ELAC or vehicle. (C) Quantification of BrdU⁺/Sox2⁺/GFAP⁺ cells within the SVZ of mice that had received i.c.v. injections of ELAC or vehicle. *P < 0.05 when comparing ELAC‐ with vehicle‐injected SVZs in a one‐way ANOVA test; #P < 0.05 when comparing ipsilateral and contralateral SVZs within the same animal group in a Student's t‐test for paired samples. Abbreviations: LV, lateral ventricle. Scale bar: 100 μm.