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Chemical synthesis pathway and permeability properties of fluorescent melatonin receptor ligands. (A) Chemical synthesis of ICOA‐9 (3) and ICOA‐13 (7). (B) Representative fluorescence microscopy images of intact (i, iii) and permeabilized (ii, iv) non‐transfected HEK293 cells incubated with 100 nM ICOA‐9 (i, ii) (green) or ICOA‐13 (iii, iv) (red). Cell nuclei are stained with DAPI (blue). Scale bar = 50 μm.
