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HPLC chromatogram of (A) two standards mixture and (B) the 80% (v/v) ethanol extracts combinational preparation PM21 at 280 nm. 5-Hydroxymethylfurfural (1) and isoorientin (2) appeared at a retention time of approximately 5.8 minutes and 21.5 minutes, respectively. PM21 contained 0.08 ± 0.01 mg/g for 5-hydroxymethylfurfural and 4.8 ± 0.07 mg/g for isoorientin. HPLC, high-performance liquid chromatography.
