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. 2017 Jun 15;8:15838. doi: 10.1038/ncomms15838

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Copyright © 2017, The Author(s)

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Quantification of MK localization in the BM reveals less sinusoidal contact (SC) and increased localization in the BM haematopoietic compartment (BMHC) in Gp1ba^−/− mice (grey) compared to the wild-type (wt, black) (n=5); intrasinusoidal (intrasin.). (b) Representative confocal images of immunostained BM of wt and Gp1ba^−/− mice. Scale bars, 50 μm. MKs, proplatelets and platelets are shown by CD41 staining in green. Endoglin staining (red) labels vessels. DAPI, blue. Arrowhead indicates MKs in the BM haematopoietic compartment (BMHC). Bar graphs represent mean±s.d. Two-way ANOVA with Bonferroni correction for multiple comparisons; **P<0.01; *P<0.05.