Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jun 15;8:15838. doi: 10.1038/ncomms15838

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017, The Author(s)

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a) Confocal images of immunostained BM and (b) TEM analysis of BM MKs of wt (left panel), Gp1ba^−/−;tg (Gp1ba-Tg) (middle panel) and wt mice after treatment with GPIbα-blocking monovalent Fab fragments, p0p/B-Fab (right panel). n=4 biological replicates. Scale bars, 50 μm (a) and 2 μm (b). MKs, proplatelets and platelets are shown by GPIX staining in green. Endoglin staining (red) labels vessels. DAPI, blue. Arrowhead indicates MKs in the BM haematopoietic compartment (BMHC). (c) Quantification of MK localization in the BM reveals less sinusoidal contact (SC) in Gp1ba-Tg (dark grey) and wt mice after GPIbα-blockade (light grey) compared to the wt (black) (n=7, 5 and 10); intrasinusoidal (intrasin.). (d,e) Reduced platelet count (d) and increased platelet size (e) in wt mice after GPIbα-blockade (n=4). (f) Representative confocal images of immunostained BM of Cdc42^−/− mice. Scale bar, 50 μm. (g) Quantification of MK localization in the BM reveals reduced SC in wt (black) and Cdc42^−/− (grey) mice (n=10 and 3). (h) TEM analysis of BM MKs of Cdc42^−/− mice. Scale bar, 2 μm. Bar graphs represent mean±s.d. (c,g) Two-way ANOVA with Bonferroni correction for multiple comparisons; (d,e) Unpaired two-tailed Student’s t-test; ***P<0.001.