Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jun 16;8:15853. doi: 10.1038/ncomms15853

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017, The Author(s)

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a) T6SS dynamics in F. novicida U112 iglA-sfGFP ΔclpB. Arrowheads indicate T6SS sheath assembly, contraction and location of sheath after contraction. Empty arrowheads indicate non-dynamic IglA-sfGFP foci. First image is a merge of phase contrast and GFP channels, following images represent GFP channel only. (b) Kymogram of F. novicida U112 iglA-sfGFP ΔclpB over 5 min (3 s per pixel). First image is a merge of phase contrast and GFP channels, following images represent GFP channel only. (c) Colocalization of ClpB-mCherry2 with IglA-sfGFP (arrows) in F. novicida U112 iglA-sfGFP clpB-mCherry2. First image is a merge of phase contrast, GFP and mCherry channels, following images represent GFP channel (upper panel) and mCherry channel (lower panel). (d) Quantification of cytosolic bacteria in unprimed wild-type BMDMs 4 h after infection with F. novicida U112 iglA-sfGFP wild type, ΔpdpB or ΔclpB (normalized to wild type). (e) Release of LDH and mature IL-1β from primed wild-type BMDMs 10 h after infection with F. novicida U112 iglA-sfGFP wild type, ΔpdpB or ΔclpB (NI—noninfected control). (a–c) 3.3 × 3.3 μm fields of view are shown. Scale bars, 1 μm. (d,e) Data are pooled from three independent experiments (d) (mean and s.d. are shown) or representatives of three independent experiments (e) (mean and s.d. of triplicate wells are shown). **P<0.01 and ****P<0.0001 (two-tailed unpaired t-test with Welch’s correction).