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Solvent gradient condition for HPLC-DAD
| Final time (min) | Flow rate (mL/min) | A | B |
|---|---|---|---|
| 0 | 0.3 | 85 | 15 |
| 10 | 0.3 | 80 | 20 |
| 30 | 0.3 | 65 | 35 |
| 35 | 0.3 | 65 | 35 |
| 40 | 0.3 | 85 | 15 |
A, water with 0.1% formic acid; B, acetonitrile with 0.1% formic acid; HPLC-DAD, high-performance liquid chromatography with diode array detection.
