Figure 8. Cancer associated ZRANB3 mutations.

(a) ZRANB3 mutations associated with endometrial carcinomas. Shown are positions of the mutations targeting conserved residues. Truncating mutations are indicated as black dots. (b) Nuclease assay with the wild-type and cancer associated mutant ZRANB3 proteins, in the presence and absence of ATP. Reactions were analysed by native polyacrylamide gel electrophoresis. Indicated are mobilities of the fluorescently labelled DNA substrate and product. (c) Quantification of the ATPase and nuclease activities of the wild-type and mutant ZRANB3 proteins. Shown are the averages of three ATPase and five nuclease reactions. s.d.’s are shown as error bars.