Table 2. Summary of lifespan experiments with mutant C. elegans strains.

C.elegans strain	E. coli strain/RNAi	Media/treatment	Mean survival, days±s.d.	P value	Mean increase/decrease, %±s.d.
daf-2	e.v.	NGM	34.83±0.35
daf-2	e.v.	NGM+glucose	29.29±0.46	0.00004	−15.91±1.16
daf-2	gsy-1	NGM	32.86±0.73	0.00678	−5.66±2.22
daf-2	gsy-1	NGM+glucose	32.95±1.31	0.03744	−5.41±3.09
daf-2	pyg-1	NGM	37.34±3.86	0.16279	7.15±10.43
daf-2	pyg-1	NGM+glucose	21.66±1.03	0.00002	−37.83±2.37
daf-16	e.v.	NGM	12.35±1.2
daf-16	e.v.	NGM+glucose	9.55±0.49	0.01001	−22.21±8.2
daf-16	gsy-1	NGM	13.95±0.88	0.06641	−13.35±8.3
daf-16	gsy-1	NGM+glucose	10.79±0.23	0.04679	−12.17±8.16
daf-2; daf-16	e.v.	NGM	13.15±1.24
daf-2; daf-16	e.v.	NGM+glucose	11.53±0.72	0.03867	−12.24±0.26
daf-2; daf-16	gsy-1	NGM	14.56±1.16	0.11200	11.02±8.25
daf-2; daf-16	gsy-1	NGM+glucose	12.28±0.19	0.10455	−6.18±8.50
aak-2	e.v.	NGM	12.76±0.16
aak-2	e.v.	NGM+glucose	10.23±0.55	0.00076	−19.85±3.35
aak-2	gsy-1	NGM	12.54±0.77	0.32458	−1.79±4.87
aak-2	gsy-1	NGM+glucose	9.89±0.32	0.00008	−22.53±2.5

The percentage of increase (+) or decrease (−) of the lifespan is shown relative to the control of the same experiment. P values were calculated with respect to control animals in the same experiment using Student’s t-test (one-tailed distribution and two-sample equal variance). See also Supplementary Table 2 for detail on individual experiments.