(A) Design of the cartridge. 1 – blister position, 2 – socket for a sample container, 3,5,6,8 – washing chambers (10 μl each), 4 – optionally washing or lysis chamber (20 μl), 7 – amplification chamber (50 μl), 9 – (optionally) hybridization chamber, 10 – waste chamber. T1–T4: positions of Peltier elements (on the external instrument). Ch1: 1.4 mm-wide channel, Ch2 – 0.4 mm-wide channel. (B) Attachment of a sample container (a syringe or a special container) to the socket.