Table 1. . Reaction mixes for the DNA amplification.

Reagent	Provider	Concentration	Volume, μl	Primer sequence, 5′–3′	Ref.
LAMP
Primer FIP-F2	Eurogentec, Germany	20 μM	2.25	GCGCGGCATCCGCATCAATA-TGCCCGGTAAACAGATGAGT	([16] for the six listed LAMP primers)
Primer BIP-B2	Eurogentec, Germany	20 μM	2.25	GCGAACGGCGAAGCGTACTG-TCGCACCGTCAAAGGAAC	–
Primer B3	Eurogentec, Germany	10 μM	0.25	CGGCAATAGCGTCACCTT	–
Primer F3	Eurogentec, Germany	10 μM	0.25	CGGCCCGATTTTCTCTGG	–
Primer Loop-F	Eurogentec, Germany	10 μM	2.5	GGCCTTCAAATCGGCATCAAT	–
Primer Loop-B	Eurogentec, Germany	10 μM	2.5	GAAAGGGAAAGCCAGCTTTACG	–
MgSO4, molecular biology grade	Sigma Aldrich, Germany	300 mM	0.5		–
dNTPs	PeqLab, Germany	10 mM each	3		–
Isothermal amplification buffer	New England Biolabs, Germany	10×	2.5		–
Bst polymerase	New England Biolabs, Germany	2 U/μl	2		–
EvaGreen™	Biotium, USA	20×	1		–
S. enteritidis DNA for positive control	Isolated with QIAamp DNA Mini Kit; Qiagen, Germany	63.9 ng/μl	2		–
Water, PCR grade	Jena Bioscience, Germany	-	to 25 μl†		–
PCR
LightCycler® 480 SYBR Green I Master Mix	Roche, Germany	2×	10		–
Forward primer uidA	Eurogentec, Germany	10 μM	1	GTTACGTCCTGTAGAAAGCCC	[17,18]
Reverse primer uidA	Eurogentec, Germany	10 μM	1	AAAACTGCCTGGCACAGCAATT	[17,18]
E. coli DNA for positive control	Isolated with QIAamp DNA Mini Kit; Qiagen, Germany	0.5 ng/μl	1		–
Water, molecular biology grade	Jena Bioscience, Germany	-	to 20 μl†		–

^†Volumes were proportionally scaled up to 200 μl to fill the cartridge lane.

LAMP: Loop-mediated isothermal amplification.