Table 3.
Stability of ZMC1 in human plasma under different storage conditions (n = 3)
| Storage conditions | Concentration (ng/mL) | Measured concentration (ng/mL) | RSD (%) | Bias (%) |
|---|---|---|---|---|
| Short-term (2 h on ice) | 500.0 | 493.9 | 3.9 | −1.3 |
| 31.3 | 29.0 | 0.7 | −7.0 | |
| 3.9 | 3.7 | 0.4 | −0.4 | |
| Long term (30 days at −80 ºC) | 500.0 | 516.4 | 4.8 | 3.2 |
| 31.3 | 30.3 | 11.2 | −2.9 | |
| 3.9 | 3.9 | 1.9 | −0.01 | |
| Three freeze– thaw cycles | 500.0 | 553.1 | 553.1 | 10.6 |
| 31.3 | 29.0 | 28.0 | −10.4 | |
| 3.9 | 4.1 | 4.1 | 4.8 | |
| Autosampler for 24 h | 500.0 | 550.7 | 1.6 | 10.0 |
| 31.3 | 35.4 | 0.7 | 13.0 | |
| 3.9 | 4.1 | 2.3 | 4.0 |