Table 2.
Comparison of the characterisation of the invertases between sugarcane and sorghum
| Sorghum | Sugarcane | Coverage/identity | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Gene name | Protein size(aa) | MW (kDa) | pI | Subcellular localization | Gene name | Protein size(aa) | MW (kDa) | pI | Subcellular localization | |||
| SignalP/Chlor-oP/MitoProt | Plant-mPLoc | SignalP/Chlor-oP/MitoProt | Plant-mPLoc | |||||||||
| SbN/AINV1 | 559 | 63.16 | 6.3 | N/44.6c/22.3m | Chloroplast | ShN/AINV1 | 559 | 63.13 | 6.1 | N/44.6c/23.8m | Chloroplast | 100%/98% |
| SbN/AINV2 | 572 | 64.17 | 6.5 | N/44.8c/3.1m | Chloroplast | ShN/AINV2–1 | 567 | 63.58 | 6.0 | N/44.3c/2.3m | Chloroplast Mitochondrion | 99%/97% |
| ShN/AINV2–2 | 575 | 64.58 | 6.5 | N/45.6c/4.1m | Chloroplast Cytoplasm | 100%/97% | ||||||
| SbN/AINV3 | 558 | 63.65 | 6.2 | N/43.4c/6.2m | Chloroplast | ShN/AINV3–1 | 557 | 63.58 | 6.2 | N/43.5c/22.1m | Chloroplast | 100%/99% |
| ShN/AINV3–2 | 557 | 63.58 | 6.2 | N/43.5c/2.1m | Chloroplast | 100%/99% | ||||||
| SbN/AINV4 | 627 | 70.51 | 7.9 | N/43.0c/0.3m | Chloroplast | ShN/AINV4–1 | 563 | 64.06 | 6.7 | N/43.1c/0.1m | Chloroplast Nucleus | 100%/98% |
| ShN/AINV4–2 | 563 | 63.90 | 6.7 | N/43.1c/0.3 m | Chloroplast | 100%/97% | ||||||
| SbN/AINV5 | 603 | 67.93 | 6.3 | N/54.9c/89.7m | Chloroplast | ShN/AINV5 | 607 | 68.28 | 6.3 | N/55.2c/97.9m | Chloroplast | 100%/97% |
| SbN/AINV6 | 627 | 70.06 | 5.4 | N/55.3c/98.6m | Chloroplast | ShN/AINV6–1 | 623 | 69.67 | 5.4 | N/53.1c/99.6m | Chloroplast | 99%/98% |
| ShN/AINV6–2 | 629 | 70.23 | 5.4 | N/54.5c/99.2m | Chloroplast | 100%/98% | ||||||
| SbCWINV1 | 579 | 63.25 | 5.9 | Y/46.2c/56.1m | Cell wall | ShCWINV1 | 572 | 62.79 | 6.2 | Y/45.7c/37.2m | Cell wall | 98%/94% |
| SbCWINV3 | 594 | 65.67 | 9.3 | N/43.8c/3.8m | Cell wall | ShCWINV3* | 453 | 50.21 | 9.4 | N/44.1c/12.2m | Cell wall | 100%/92% |
| SbCWINV6 | 599 | 66.90 | 9.5 | N/44.5c/2.5m | Cell wall | ShCWINV6* | 349 | 39.84 | 9.5 | N/44.0c/53.5m | Cell wall | 66%/90% |
| SbCWINV7 | 643 | 73.06 | 6.7 | Y/50.9c/87.6m | Cell wall | ShCWINV7–1 | 588 | 66.40 | 6.4 | Y/50.4c/72.9m | Cell wall | 99%/90% |
| ShCWINV7–2 | 598 | 67.84 | 6.2 | Y/50.7c/69.2m | Cell wall | 99%/90% | ||||||
| ShCWINV7–3 | 593 | 67.13 | 6.3 | Y/51.1c/85.3m | Cell wall | 99%/90% | ||||||
| SbCWINV8 | 556 | 61.44 | 5.4 | N/44.6c/49.4m | Cell wall | ShCWINV8–1* | 501 | 55.60 | 5.4 | N/44.1c/41.5m | Cell wall | 88%/92% |
| ShCWINV8–2* | 448 | 49.46 | 5.0 | N/46.7c/9.4m | Cell wall | 76%/93% | ||||||
| SbCWINV9 | 590 | 64.62 | 5.3 | Y/46.3c/9.7m | Cell wall | ShCWINV9–1* | 211 | 23.08 | 5.3 | N/44.3c/8.9m | Cell wall | 40%/94% |
| ShCWINV9–2* | 503 | 54.76 | 4.9 | N/44.6c/5.3m | Cell wall | 89%/89% | ||||||
| ShCWINV9–3 | 574 | 62.60 | 5.1 | Y/48.1c/9.1m | Cell wall | 100%/91% | ||||||
| SbCWINV10 | 625 | 68.30 | 5.2 | Y/51.5c/25.3m | Cell wall | ShCWINV10 | 592 | 64.39 | 6.0 | Y/50.1c/0.5m | Cell wall | 99%/87% |
| SbVINV1 | 638 | 69.32 | 5.7 | N/46.3c/8.3m | Vacuole | ShVINV1* | 487 | 52.94 | 5.0 | N/46.3c/5.0m | Vacuole | 100%/94% |
Note: m Probability (%) of targeting to mitochondrion, c Probability (%) of targeting to chloroplast; N--Non-secretory protein, Y-- Secretory protein, * represented truncated gene