Table 4.
Chemical composition of brothsa and pig and calf sera: mean and standard deviation (SD) (n = 3 batches)a
| Matrix | Total protein (g/L) | Albumin (g/L) | Globulin (g/L) | Sodium (mmol/L) | Potassium (mmol/L) | Calcium (mmol/L) | Magnesium (mmol/L) | Chloride (mmol/L) | Inorganic phosphorus (mmol/L) |
Urea (mmol/L) | Creatinine (μmol/L) | Glucose (mmol/L) | Iron (μmol/L) | Unsaturated Iron Binding Capacity (μmol/L) |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Porcine serum | 79.3 | 40.3 | 39.0 | 153 | 7.13 | 2.59 | 0.98 | 115 | 2.82 | 4.67 | 139 | 5.47 | 19.1 | 78.00 |
| SD | 2.20 | 0.17 | 2.03 | 4.4 | 0.06 | 0.14 | 0.09 | 8.96 | 0.61 | 3.35 | 5.5 | 0.84 | 0.15 | 0.36 |
| Bovine serum | 74.3 | 38.1 | 37.1 | 148 | 7.07 | 2.55 | 1.00 | 108 | 3.28 | 5.23 | 149 | 4.43 | 22.2 | 71.00 |
| SD | 3.65 | 2.11 | 1.31 | 15.4 | 1.04 | 0.55 | 0.17 | 7.55 | 0.98 | 0.60 | 9.9 | 0.29 | 0.61 | 0.43 |
| MHB | 3.23 | 0.00 | 3.23 | 125 | 2.49 | 0.13 | 0.23 | 99.23 | 2.55 | 0.93 | 61.3 | 2.57 | 9.47 | -6.90 |
| SD | 0.12 | 0.00 | 0.12 | 0.2 | 0.01 | 0.02 | 0.00 | 0.32 | 0.03 | 0.06 | 2.31 | 0.06 | 0.15 | 0.44 |
| CAMHB | 4.10 | 0.07 | 4.03 | 127 | 2.87 | 5.21 | 4.20 | 117 | 2.96 | 2.97 | 83.3 | 2.57 | 9.53 | -7.20 |
| SD | 0.44 | 0.06 | 0.46 | 11.5 | 0.21 | 2.14 | 1.68 | 5.29 | 0.34 | 0.64 | 2.08 | 0.25 | 0.17 | 1.24 |
| CB | 12.6 | 0.10 | 12.5 | 130 | 14.58 | 0.22 | 1.41 | 112 | 4.99 | 2.90 | 217 | 12.90 | 63.9 | -56.50 |
| SD | 0.20 | 0.00 | 0.20 | 0.1 | 0.01 | 0.01 | 0.02 | 0.26 | 0.02 | 0.10 | 2.31 | 0.00 | 0.62 | 1.74 |
| BHI | 9.67 | 0.10 | 9.57 | 168 | 10.01 | 0.13 | 0.25 | 123 | 6.86 | 5.97 | 418 | 10.20 | 7.50 | -3.17 |
| SD | 0.21 | 0.00 | 0.21 | 0.2 | 0.01 | 0.01 | 0.01 | 0.56 | 0.07 | 0.06 | 3.61 | 0.20 | 0.10 | 1.11 |
| TSB | 9.07 | 0.10 | 9.00 | 118 | 9.56 | 0.05 | 0.18 | 79.33 | 4.85 | 2.17 | 260 | 11.77 | 6.86 | -2.20 |
| SD | 0.06 | 0.06 | 0.10 | 0.1 | 0.01 | 0.02 | 0.00 | 0.06 | 0.02 | 0.06 | 1.53 | 0.15 | 0.25 | 1.74 |
| VFM | 11.4 | 0.87 | 10.5 | 132 | 38.73 | 0.33 | 1.16 | 104 | 14.31 | 4.87 | 198 | 3.40 | 25.9 | -3.00 |
| SD | 0.26 | 0.12 | 0.15 | 3.5 | 0.31 | 0.03 | 0.10 | 4.04 | 0.44 | 0.72 | 9.07 | 0.10 | 0.52 | 1.31 |
aMueller Hinton Broth (MHB), Cation Adjusted Mueller Hinton Broth (CAMHB),, Veterinary Fastidious Medium (VFM), Columbia Broth supplemented with NAD (CB), Brain Heart Infusion broth (BHIB), Tryptic Soy broth (TSB) and Veterinary Fastidious Medium (VFM)