Table 2.
Results for generation of liver orthotopic patient-derived xenograft mouse models and their tumor characteristics
| Case | Patient tumor (X0) | Xenograft (X1) | Xenograft (X2) | Xenograft (X3) | Pigmentation | Ki67% positive cells (%) | Monosomy 3a | c-myc copy numbera | GNAQ | GNA11 | BAP1 | SF3B1 | EIF1AX | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Sex | Age | Sample | Engraftment | Tumor take rate (mouse) | Volume (mm3) (median, range) | Engraftment | Engraftment | X0 | X1 | X0 | X1 | X0 | X1 | X0 | X1 | X0 | X1 | X0 | X1 | X0 | X1 | X0 | X1 | X0 | X1 | |
| 1 | F | 59 | Surgery | Success | 3/3 | 272.2, 240.3–344.6 | Success | Success | Moderate | Moderate | 20.7 | 16.1 | + | + | Large | Large | – | – | – | – | + | + | – | – | – | – |
| 2 | F | 41 | Biopsy | Success | 2/2 | 931.8, 831.7–1031.9 | Success | Success | None | None | 20.8 | 20.1 | + | + | Large | Large | + | + | – | – | – | – | – | – | – | – |
| 3 | M | 67 | Biopsy | Failure | 0/3 | NA | NA | NA | None | NA | IS | NA | + | NA | Large | NA | IS | NA | IS | NA | IS | NA | IS | NA | IS | NA |
| 4 | M | 70 | Biopsy | Success | 2/2 | 86.73, 60.0–113.4 | Success | Success | Moderate | Moderate | 10.0 | 14.3 | + | + | Large | Large | + | + | – | – | – | – | – | – | – | – |
| 5 | F | 59 | Biopsy | Success | 2/2 | 835.3, 572.9–1097.7 | Success | Success | None | None | 20.6 | 24.6 | + | + | Large | Large | – | – | + | + | + | + | – | – | – | – |
| 6 | F | 62 | Biopsy | Failure | 0/2 | NA | NA | NA | Heavy | NA | 0 | NA | – | NA | Small | NA | – | NA | – | NA | – | NA | + | NA | – | NA |
| 7 | M | 66 | Biopsy | Success | 3/3 | 35.3, 28.2–199.1 | Success | Success | None | Minimal | 13.5 | 16.1 | + | + | Small | Small | – | – | + | + | + | + | – | – | – | – |
| 8 | M | 73 | Biopsy | Success | 2/2 | 137.8, 123.0–152.6 | Failure | NA | None | Minimal | 3.5 | 4.5 | IS | + | IS | Large | IS | IS | IS | IS | IS | IS | IS | IS | IS | IS |
| 9 | M | 71 | Surgery | Success | 1/4 | 36.1 | Failure | NA | Moderate | Moderate | 7.2 | 3.9 | + | + | Large | Large | – | – | + | UR | + | + | – | – | – | – |
| 10 | F | 49 | Biopsy | Success | 1/1 | 6.61 | Failure | NA | Minimal | Minimal | 3.0 | 6.9 | – | – | Small | Small | + | + | – | – | – | – | + | + | – | – |
| 11 | F | 52 | Biopsy | Success | 2/3 | 5.08, 4.85–5.32 | Failure | NA | Moderate | Moderate | 1.3 | 0 | IS | + | IS | Small | – | – | – | – | – | – | – | – | – | + |
| 12 | M | 48 | Biopsy | Success | 1/1 | 1123.7 | Success | Success | Moderate | Moderate | 13.7 | 18.3 | + | + | Large | Large | + | + | – | – | + | + | – | – | – | – |
X 0 patient’s tumor, X 1 first-generation mice, X 2 second-generation mice, X 3 third-generation mice, F female, M male, NA not applicable, IS insufficient samples, UR unreadable due to low coverage of this lesion, none no pigmentation, minimal one-third or less pigmented, moderate one-third to two-thirds pigmented, Heavy two-thirds to complete pigmented, large 4 copies, small 3 copies or partial 4 copies
aMonosomy 3 and c-myc gain were evaluated by copy number variation analysis