Table 1.
Details of the primary antibodies used in this study and the requirement for antigen retrieval (pepsin treatment)a
| Antigen | Species | Source | Number | Dilution (Western) | Dilution (IHC) | Pepsin (IHC) |
|---|---|---|---|---|---|---|
| γ-2 | Guinea pig | M. Watanabe | — | 1:500 | — | — |
| γ-2b | Rabbit | Cell Signaling Technology | 8511 | — | 1:200 | Yesc |
| γ-3 | Guinea pig | M. Watanabe | — | 1:500 | — | — |
| γ-4 | Rabbit | Abcam | ab81107 | 1:500 | — | — |
| γ-5 | Rabbit | M. Watanabe | — | 1:500 | — | — |
| γ-7 | Rabbit | Frontiers Institute | Af720 | 1:500 | 1:500 | No |
| γ-8 | Rabbit | Frontiers Institute | Af1000 | 1:2000 | 1:1000 | No |
| GluA1 | Rabbit | Abcam | ab31232 | — | 1:500 | Yes |
| GluA2 | Mouse | Millipore | MAB397 | 1:500 | 1:500 | Yes |
| c-Fos | Goat | Santa Cruz Biotechnology | sc-52-G | — | 1:100 | No |
| Actin | Mouse | Abcam | ab3280 | 1:5000 | — | — |
| GFP | Chicken | Abcam | Ab13970 | — | 1:500 | No |
| CGRPb | Mouse | Sigma | C7113 | — | 1:400 | No |
| vGluT1b | Guinea pig | Synaptic Systems | 135 304 | — | 1:400 | No |
| vGluT2b | Guinea pig | Millipore | AB2251 | — | 1:4000 | No |
aIHC, Immunohistochemistry.
bUsed for imaging synapses (see Materials and Methods).
cPepsin used for γ-2 labeling of PFA fixed tissue but not with carbodiimide fix (synaptic imaging).