Fig. 4.

Phylogenetic tree showing the position of E. canis amplified from R. bursa compared to other Anaplasmataceae species. Evolutionary analyses were conducted using MEGA7 [32]. The concatenated 23S rRNA and the groEl genes of the Ehrlichia canis amplified in this study together with other sequences of Anaplasmataceae species available on GenBank. The evolutionary history was. A discrete Gamma distribution was used to model evolutionary rate differences among sites [4 categories (+G, parameter = 0.6567)]. The analysis involved 15 nucleotide sequences. All positions containing gaps and missing data were eliminated. There was a total of 1039 positions in the final dataset. The scale-bar represents a 5% nucleotide sequence divergence